|First Appearance||Winx Club: The Secret of the Lost Kingdom|
Mandragora is the villain of the movie The Secret of the Lost Kingdom. She is the Witch of Insects and the servant of the Ancestral Witches, blocked like them in the realm of Obsidian, of which she guards the gate, the Obsidian Circle. When the sword of Oritel awakes again in the Obsidian dimension, the three witches give Mandragora more power so that she may escape form the Obsidian Dimension, since they are themselves unable of leaving it, and search for the keeper of the Dragon Flame. First arriving Domino, where she senses the presence of the keeper of the Dragon Flame, she causes one of her insects to bite Riven, thus injecting a poison in him which enabled her to control him.
Later she attacks Alfea, which is almost completely destroyed. Finally, with the Winx' Miss Faragonda is able to defeat her and send her back to the Obsidian Circle. However, having found the keeper of the Dragon's Flame and learned her name when Faragonda called Bloom by her name before banishing Mandragora, she informed her mistresses, who immediately hatched a plot to destroy Bloom, Domino, and steal the Dragon Fire. Finally, when the Winx and the Specialists arrived at Obsidian, Mandragora attacked the boys and, manipulating Riven, cause him to remove the key from the lock of the Obsidian dimension, therefore blocking the Winx, minus Musa, inside the dark Dimension, and the Specialists and Musa outside it.
Still under her control, Riven attacked Sky, causing Musa to be harmed in an attempt to save Sky. With nearly all of her enemies disarmed, she trapped the Specialists, except Riven, in traps made of a jelly-like substance. Riven, seeing an injured Musa on the ground, remembered his love for the fairy, and the poison of Mandragora's insect was forced out of his body by his sorrow. He then attacked Mandragora, freeing the other Specialists, and all the Specialists destroyed Mandragora's insects. At the same moments, the Ancestral Witches called Mandragora back to help them. Her three mistresses possessed her in order to be able to harm Bloom, but Sky, ran the magic sword of Oritel through her, while Bloom used her Dragon Fire to burn her, finally killing her, defeating (and unknowingly freeing) the Witches and destroying the Obsidian Dimension.
From Mandragora's reactions, those of the Three Ancient Witches and of the members of the Company of Light it can be presumed that she was together with the Ancestral Witches and Valtor during the destruction of Domino, as she seems to closely know Faragonda, Hagen, as well as other members of the Company of Light. Moreover, Faragonda and Hagen also mention her by her name, and seem to have much knowledge about her. She probably used to be a server of Lord Darkar like the Ancestresses.She is the reason for Bloom's complete Enchantix.
- Mandragora's name comes from the word Mandragora, which is both the scientific and Italian name from the Mandrake plant.